bsm-52559R
[Primary Antibody]
RALBP1 (36H1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: RALBP1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 10928
Swiss Prot: Q15311
Source: C terminal Human RALBP1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of 293 cells with RALBP1 (36H1) Monoclonal Antibody (bsm-52559R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: HCT116 Cells; Lane 2: Jurkat Cells; Lane 3: 293T Cells; Probed with RALBP1 (36H1) Monoclonal Antibody (bsm-52559R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with RALBP1 (36H1) Monoclonal Antibody (bsm-52559R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with RALBP1 (36H1) Monoclonal Antibody (bsm-52559R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with RALBP1 (36H1) Monoclonal Antibody (bsm-52559R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse pancreas tissue incubated with RALBP1 (36H1) Monoclonal Antibody (bsm-52559R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with RALBP1 (36H1) Monoclonal Antibody (bsm-52559R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.