DATASHEET
Host:
Rabbit
Target Protein:
Ferritin
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
2512
Swiss Prot:
P02792
Source:
Recombinant protein within Human Ferritin Heavy Chain between 150-250 amino acids
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
VALIDATION IMAGES
Zebrafish lysate probed with Ferritin (7A5) Monoclonal Antibody (bsm-52548R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Ferritin (7A5) Monoclonal Antibody (bsm-52548R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Ferritin (7A5) Monoclonal Antibody (bsm-52548R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.