bsm-52542R [Primary Antibody]
MTCO2 (6C9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MTCO2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4513

Swiss Prot: P00403

Source: Human MTCO2 aa 200-300

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HepG2 cells with MTCO2 (6C9) Monoclonal Antibody (bsm-52542R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: MCF-7 Cells; Lane 2: THP-1 Cells; Lane 3: Hela Cells; Probed with MTCO2 (6C9) Monoclonal Antibody (bsm-52542R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with MTCO2 (6C9) Monoclonal Antibody (bsm-52542R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with MTCO2 (6C9) Monoclonal Antibody (bsm-52542R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with MTCO2 (6C9) Monoclonal Antibody (bsm-52542R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.