VALIDATION IMAGES
Flow cytometric analysis of HepG2 cells with Topoisomerase I (1D1) Monoclonal Antibody (bsm-52531R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: HepG2 Cells; Lane 2: Jurkat Cells; Lane 3: MCF-7 Cells; Probed with Topoisomerase I (1D1) Monoclonal Antibody (bsm-52531R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Topoisomerase I (1D1) Monoclonal Antibody (bsm-52531R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Topoisomerase I (1D1) Monoclonal Antibody (bsm-52531R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Topoisomerase I (1D1) Monoclonal Antibody (bsm-52531R) at 1:300 in 293 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Topoisomerase I (1D1) Monoclonal Antibody (bsm-52531R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.