bsm-52505R [Primary Antibody]
Prion Protein (PrP) (33A5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Prion Protein (PrP)

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5621

Swiss Prot: P04156

Source: C terminal human Prion protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The function of PrP is still under debate. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis (By similarity). Isoform 2 may act as a growth suppressor by arresting the cell cycle at the G0/G1 phase. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro).

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of SH-Sy5Y cells with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Rat brain; Lane 2: Mouse brain; Probed with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C


IF(ICC) staining with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.