bsm-52497R
[Primary Antibody]
Cytochrome C (8D2) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Cytochrome C
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 54205
Swiss Prot: P99999
Source: Full length protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Mouse kidney; Lane 2: Rat kidney; Probed with Cytochrome C (8D2) Monoclonal Antibody (bsm-52497R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Cytochrome C (8D2) Monoclonal Antibody (bsm-52497R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Cytochrome C (8D2) Monoclonal Antibody (bsm-52497R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with Cytochrome C (8D2) Monoclonal Antibody (bsm-52497R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Cytochrome C (8D2) Monoclonal Antibody (bsm-52497R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Cytochrome C (8D2) Monoclonal Antibody (bsm-52497R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.