VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with CD44 (8D4) Monoclonal Antibody (bsm-52484R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela cells; Lane 2: Jurkat cells; Probed with CD44 (8D4) Monoclonal Antibody (bsm-52484R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CD44 (8D4) Monoclonal Antibody (bsm-52484R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with CD44 (8D4) Monoclonal Antibody (bsm-52484R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD44 (8D4) Monoclonal Antibody (bsm-52484R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.