bsm-52478R
[Primary Antibody]
COL1A1/Collagen I Recombinant Antibody
DATASHEET
Host: Rabbit
Target Protein: COL1A1/Collagen I
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 1277
Swiss Prot: P02452
Source: Synthetic peptide of human Collagen I between 1200 to 1300 amino acids
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 130
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Jinxiu Yu. et al. Promoting osseointegration of titanium by pH-responsive releasing of H2S and optimizing polarization time for macrophages. COMPOS PART B-ENG. 2023 Mar;253:110554Read more>>
- Xiaoliang Zhou. et al. Ursolic acid inhibits human dermal fibroblasts hyperproliferation, migration, and collagen deposition induced by TGF- via regulating the Smad2/3 pathway. GENE. 2023 May;867:147367Read more>>
- Yiyang Fan. et al. Effects and Significance of Dicliptera chinensis Polysaccharide on the Expression of Transforming Growth Factor 1/Connective Tissue Growth Factor Pathway in the Masseter and Head and Neck Skin of Rats With Radiation-Induced Fibrosis. INT DENT J. 2024 JulRead more>>
VALIDATION IMAGES
Human placenta lysate probed with COL1A1/Collagen Ⅰ (5F2) Monoclonal Antibody (bsm-52478R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with COL1A1/Collagen Ⅰ (5F2) Monoclonal Antibody (bsm-52478R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Uterus tissue incubated with COL1A1/Collagen Ⅰ (5F2) Monoclonal Antibody (bsm-52478R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COL1A1/Collagen I (5F2) Monoclonal Antibody, Unconjugated (bsm-52478R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COL1A1/Collagen I (5F2) Monoclonal Antibody, Unconjugated (bsm-52478R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human esophageal carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COL1A1/Collagen I (5F2) Monoclonal Antibody, Unconjugated (bsm-52478R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Collagen I) Monoclonal Antibody, Unconjugated (bsm-52478R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Collagen I) Monoclonal Antibody, Unconjugated (bsm-52478R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated(bsm-52478R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.