VALIDATION IMAGES
Lane 1: Human liver; Lane 2: Human serum; Probed with Vitronectin (7A1) Monoclonal Antibody (bsm-52460R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Vitronectin (7A1) Monoclonal Antibody (bsm-52460R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Vitronectin (7A1) Monoclonal Antibody (bsm-52460R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Vitronectin (7A1) Monoclonal Antibody (bsm-52460R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Vitronectin (7A1) Monoclonal Antibody (bsm-52460R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with Vitronectin (7A1) Monoclonal Antibody (bsm-52460R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with Vitronectin (7A1) Monoclonal Antibody (bsm-52460R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Vitronectin (7A1) Monoclonal Antibody (bsm-52460R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.