bsm-52459R [Primary Antibody]
beta Arrestin 1 (3A11) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: beta Arrestin 1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 408

Swiss Prot: P49407

Source: Human beta Arrestin 1 between 1 to 200 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The members of the G protein coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. Members of the_-Arrestin family regulate receptor binding to G proteins. _-Arrestins have been found to be located at postsynaptic sites, where they are thought to act in concert with_ARK (_ARK1, also designated GRK 2, or_ARK2, also designated GRK 3 to regulate G protein-coupled neurotransmitter receptors. Expression of_-Arrestin-1 and b-Arrestin-2 is seen predominantly in spleen and neuronal tissues. It has been shown that_-Arrestin-1 expression is modulated by intracellular cAMP, which may be a novel mechanism for the regulation of receptor-mediated responses.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: PC12 cells; Lane 2: Jurkat cells; Probed with beta Arrestin 1 (3A11) Monoclonal Antibody (bsm-52459R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with beta Arrestin 1 (3A11) Monoclonal Antibody (bsm-52459R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with beta Arrestin 1 (3A11) Monoclonal Antibody (bsm-52459R) at 1:100 in PC-12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with beta Arrestin 1 (3A11) Monoclonal Antibody (bsm-52459R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with beta Arrestin 1 (3A11) Monoclonal Antibody (bsm-52459R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.