VALIDATION IMAGES
Lane 1: SHG-44 cells; Lane 2: Mouse brain; Lane 3: Mouse liver; Lane 4: Mouse skeletal muscle; Probed with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100 in PC-12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.