bsm-52450R
[Primary Antibody]
MAP1LC3A (9A7) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MAP1LC3A
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 84557
Swiss Prot: Q9H492
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: SHG-44 cells; Lane 2: Mouse brain; Lane 3: Mouse liver; Lane 4: Mouse skeletal muscle; Probed with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100 in PC-12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.