bsm-52439R
[Primary Antibody]
active+pro Caspase 3 (23C9) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: active+pro Caspase 3
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 836
Swiss Prot: P42574
Source: Recombinant protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Jurkat cell lysate probed with active+pro Caspase 3 (23C9) Monoclonal Antibody (bsm-52439R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with active+pro Caspase 3 (23C9) Monoclonal Antibody (bsm-52439R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with active+pro Caspase 3 (23C9) Monoclonal Antibody (bsm-52439R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.