Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: MCF-7, 2: JAR Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 46 kDa Observed MW: 70-80/85 kDa


Tissue: Human placenta Section type: Formalin-fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:2000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-52422R


IP of MCF-7 cells extracts IP ab incubation condition: PTM-6392, 4°C overnight, 1:100 dilution WB primary ab incubation condition: bsm-52422R, room temperature 2h, 1:1000 dilution Secondary ab: Anti-Rabbit IgG for IP (HRP) Blocking buffer and concentration: 5% NFDM/TBST Diluting buffer and concentration: 5% NFDM/TBST 1: 5% Input 2: IP with bsm-52422R 3: IP with Rabbit monoclonal IgG Isotype Observed MW: 70-80/85 kDa Exposure time: 120 s