VALIDATION IMAGES
Mouse brain lysate probed with DARPP32 (6A12) Monoclonal Antibody (bsm-52416R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of HeLa cells with DARPP32 (6A12) Monoclonal Antibody (bsm-52416R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with DARPP32 (6A12) Monoclonal Antibody (bsm-52416R) at 1:100 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with DARPP32 (6A12) Monoclonal Antibody (bsm-52416R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with DARPP32 (6A12) Monoclonal Antibody (bsm-52416R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with DARPP32 (6A12) Monoclonal Antibody (bsm-52416R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with DARPP32 (6A12) Monoclonal Antibody (bsm-52416R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.