VALIDATION IMAGES
Lane 1: Raji cell ; Lane 2: Jurkat cell; Lane 3: A431 cell; Probed with Bim (42C9) Monoclonal Antibody (bsm-52409R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Bim (42C9) Monoclonal Antibody (bsm-52409R) at 1:100 in HeLa cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Bim (42C9) Monoclonal Antibody (bsm-52409R) at 1:100 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Bim (42C9) Monoclonal Antibody (bsm-52409R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PKC alpha (2A1) Monoclonal Antibody (bsm-52409R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with PKC alpha (2A1) Monoclonal Antibody (bsm-52409R) at 1:100 in A549 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse intestine tissue incubated with PKC alpha (2A1) Monoclonal Antibody (bsm-52409R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with PKC alpha (2A1) Monoclonal Antibody (bsm-52409R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with PKC alpha (2A1) Monoclonal Antibody (bsm-52409R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.