VALIDATION IMAGES
Lane 1: BT-20 cell lysates; Lane 2: HUVEC cell lysates; Lane 3: SW480 cell lysates probed with HAPLN1 (30A1) Monoclonal Antibody, Unconjugated (bsm-52402R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
IF(ICC) staining with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100 in BT-20 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.