bsm-52396R
[Primary Antibody]
ACTA2 (2B10) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ACTA2
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 59
Swiss Prot: P62736
Source: Recombinant human ACTA2 between 1 to 200
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 42
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Chih-Hsin Hsu. et al. miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis. Int J Mol Sci. 2021 Jan;22(19):10574Read more>>
- Shen, Zhou. et al. Bone marrow mesenchymal stem cells therapy on bilateral pelvic nerve crush-induced voiding dysfunction in rats. INT UROGYNECOL J. 2022 Apr;:1-8Read more>>
- Zhang, Mingming. et al. Both high glucose and phosphate overload promote senescence-associated calcification of vascular muscle cells. INT UROL NEPHROL. Int Urol Nephrol. 2022 Apr;:1-13Read more>>
- Yingchun Luo. et al. Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis. EBIOMEDICINE. 2022 Aug;82:104087Read more>>
- Ya-Ling Yin. et al. Citronellal Attenuates Oxidative Stress–Induced Mitochondrial Damage through TRPM2/NHE1 Pathway and Effectively Inhibits Endothelial Dysfunction in Type 2 Diabetes Mellitus. ANTIOXIDANTS-BASEL. 2022 Nov;11(11):2241Read more>>
- Lu Tan. et al. Mechanically Robust Hemostatic Hydrogel Membranes with Programmable Strain-Adaptive Microdomain Entanglement for Wound Treatment in Dynamic Tissues. ACS NANO. 2024;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES
Lane 1: Hela cell lysates;Lane 2: A431 cell lysates; Lane 3: NIH/3T3 cell lysates probed with alpha smooth muscle Actin (2B10) Monoclonal Antibody (bsm-52396R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
IF(ICC) staining with alpha smooth muscle Actin (2B10) Monoclonal Antibody (bsm-52396R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with alpha smooth muscle Actin (2B10) Monoclonal Antibody (bsm-52396R) at 1:300 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human stomach carcinoma tissue incubated with alpha smooth muscle Actin (2B10) Monoclonal Antibody (bsm-52396R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Jurkat cells with ACTA2 (2B10) Monoclonal Antibody (bsm-52396R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with ACTA2 (2B10) Monoclonal Antibody (bsm-52396R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with ACTA2 (2B10) Monoclonal Antibody (bsm-52396R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with ACTA2 (2B10) Monoclonal Antibody (bsm-52396R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with ACTA2 (2B10) Monoclonal Antibody (bsm-52396R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with ACTA2 (2B10) Monoclonal Antibody (bsm-52396R) at 1:100 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with ACTA2 (2B10) Monoclonal Antibody (bsm-52396R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.