VALIDATION IMAGES
Lane 1:Human skeletal muscle lysates; Lane 2: Mouse heart lysates probed with Desmin (5A5) Monoclonal Antibody, Unconjugated (bsm-52363R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
IF(ICC) staining with Desmin (5A5) Monoclonal Antibody (bsm-52363R) at 1:100 in C2C12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with Desmin (5A5) Monoclonal Antibody (bsm-52363R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse bladder tissue incubated with Desmin (5A5) Monoclonal Antibody (bsm-52363R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Desmin (5A5) Monoclonal Antibody (bsm-52363R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse skeletal muscle tissue incubated with Desmin (5A5) Monoclonal Antibody (bsm-52363R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.