VALIDATION IMAGES
COS-1 cell lysates probed with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
IF(ICC) staining with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.