bsm-52352R [Primary Antibody]
Hsp90 beta (1A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Hsp90 beta

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3326

Swiss Prot: P08238

Source: Recombinant protein within Human Hsp90 beta aa 500 to the C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 83 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: K562 cell lysates probed with Hsp90 beta (1A1) Monoclonal Antibody (bsm-52352R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.


Flow cytometric analysis of Jurkat cells probed with Hsp90 beta (1A1) Monoclonal Antibody (bsm-52352R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


IF(ICC) staining with Hsp90 beta (1A1) Monoclonal Antibody (bsm-52352R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Hsp90 beta (1A1) Monoclonal Antibody (bsm-52352R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Hsp90 beta (1A1) Monoclonal Antibody (bsm-52352R) at 1:300 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Hsp90 beta (1A1) Monoclonal Antibody (bsm-52352R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Hsp90 beta (1A1) Monoclonal Antibody (bsm-52352R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Hsp90 beta (1A1) Monoclonal Antibody (bsm-52352R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Hsp90 beta (1A1) Monoclonal Antibody (bsm-52352R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Hsp90 beta (1A1) Monoclonal Antibody (bsm-52352R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Hsp90 beta (1A1) Monoclonal Antibody (bsm-52352R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.