bsm-52348R [Primary Antibody]
CDX2 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CDX2

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 1045

Swiss Prot: Q99626

Source: Recombinant human CDX2 between 1 to 150 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Rat
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

AGS cell lysates probed with CDX2 (3G7) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.


Flow cytometric analysis of HeLa lysate probed with CDX2 (3G7) Monoclonal Antibody (bsm-52348R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


IF(ICC) staining with CDX2 (3G7) Monoclonal Antibody (bsm-52348R) at 1:100 in AGS cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with CDX2 (3G7) Monoclonal Antibody (bsm-52348R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat large intestine tissue incubated with CDX2 (3G7) Monoclonal Antibody (bsm-52348R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.