bsm-52303R
[Primary Antibody]
Caveolin-1 (9C11) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CAV1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 857
Swiss Prot: Q03135
Source: Human Caveolin-1 aa 100-250
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
IF(ICC) staining with Caveolin-1 (9C11) Monoclonal Antibody (bsm-52303R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Caveolin-1 (9C11) Monoclonal Antibody (bsm-52303R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Caveolin-1 (9C11) Monoclonal Antibody (bsm-52303R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Caveolin-1 (9C11) Monoclonal Antibody (bsm-52303R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Caveolin-1 (9C11) Monoclonal Antibody (bsm-52303R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Caveolin-1 (9C11) Monoclonal Antibody (bsm-52303R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Caveolin-1 (9C11) Monoclonal Antibody (bsm-52303R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with Caveolin-1 (9C11) Monoclonal Antibody (bsm-52303R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: A549 Cells; Probed with Caveolin-1 (9C11) Monoclonal Antibody (bsm-52303R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.