bsm-52280R [Primary Antibody]
MAP2K1/2 (4H7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MAP2K1/2

Specificity: The antibody will detect both MAP2K1 and MAP2K2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5604; 5605

Swiss Prot: Q02750; P36507

Source: Human MAP2K1 + MAP2K2 aa 1 to the C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Mouse
Rat
Fish
Zebrafish
(Crucian carp (Carassius carassius))

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HepG2; Lane 2: PC12; Lane 3: NIH/3T3 probed with MAP2K1/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with MAP2K1/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with MAP2K1/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with MAP2K1/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with MAP2K1/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with MAP2K1/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Crucian carp (Carassius carassius) Tissue; Probed with MAP2K1/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.