bsm-52274R [Primary Antibody]
CCR7 (1G8) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CCR7

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1236

Swiss Prot: P32248

Source: Recombinanat human CCR7 between 1 to 100 amino acids.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

CCR7 is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. Signals mediated by this receptor regulate T cell homeostasis in lymph nodes, and may also function in the activation and polarization of T cells, and in chronic inflammation pathogenesis. Alternative splicing of this gene results in multiple transcript variants.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

IF(ICC) staining with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Mouse Lymph node tissue lysates; Lane 4: Mouse Lung tissue lysates ; Lane 5: Rat Thymus tissue lysates;Lane 6:Rat Spleen tissue lysates; Lane 7:Rat Lymph node tissue lysates; Lane 8: Rat Lung tissue lysates; Lane 9: Human Jurkat cell lysates; Lane 10: Human MCF-7 cell lysates; Lane 11: Human Hela cell lysates ; Lane 12: Human HL60 cell lysates probed with CCR7 (1G8) Monoclonal Antibody, Unconjugated (bsm-52274R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.