bsm-52271R [Primary Antibody]
PTPN6 (1A5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PTPN6

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5777

Swiss Prot: P29350

Source: Human PTPN6 from 550 amino acids to C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 68


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Raji cell lysates probed with PTPN6 (1A5) Monoclonal Antibody (bsm-52271R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of HeLa cells with PTPN6 (1A5) Monoclonal Antibody (bsm-52271R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).


IF(ICC) staining with PTPN6 (1A5) Monoclonal Antibody (bsm-52271R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with PTPN6 (1A5) Monoclonal Antibody (bsm-52271R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with PTPN6 (1A5) Monoclonal Antibody (bsm-52271R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PTPN6 (1A5) Monoclonal Antibody (bsm-52271R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat spleen tissue incubated with PTPN6 (1A5) Monoclonal Antibody (bsm-52271R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse spleen tissue incubated with PTPN6 (1A5) Monoclonal Antibody (bsm-52271R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with PTPN6 (1A5) Monoclonal Antibody (bsm-52271R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with PTPN6 (1A5) Monoclonal Antibody (bsm-52271R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.