bsm-52258R
[Primary Antibody]
JNK1+JNK2+JNK3 (3G7) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: JNK1+JNK2+JNK3
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: P45983, P45984, P53779
Source: Human JNK1+JNK2+JNK3 between 1 to 400 amino acids
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1a1,JNK1b1, JNK2a1, JNK2b1, and JNK3a1, which represent the p46 isoforms, and JNK1a2, JNK1b2, JNK2a2, JNK2b2, and JNK3b2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
Monkey
Zebrafish
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Hela; Lane 2: PC12; Lane 3: K562 lysate probed with JNK1+JNK2+JNK3 (3G7) Monoclonal Antibody (bsm-52258R) overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with JNK1+JNK2+JNK3 (3G7) Monoclonal Antibody (bsm-52258R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.