Host: Rabbit
Target Protein: STAT5b
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 6777
Swiss Prot: P51692
Source: Recombinant human STAT5b protein, around 500-600aa.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: K562; Lane 2: Hela lysate probed with STAT5b (12A1) Monoclonal Antibody (bsm-52238R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded Human spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT5b (12A1) Monoclonal Antibody (bsm-52238R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT5b (12A1) Monoclonal Antibody (bsm-52238R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.