bsm-52213R [Primary Antibody]
NTRK2 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NTRK2

Modification Site: Y817

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4915

Swiss Prot: Q16620

Source: KLH conjugated Synthesised phosphopeptide derived from human NTRK2 around the phosphorylation site of Tyr817:PV(p-Y)LD

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jun Wang. et al. Network pharmacology analysis of the active ingredients of Corydalis hendersonii Hemsl. and their effects on eliminating neuroinflammation and improving motor functions in MPTP-intoxicated mice. J ETHNOPHARMACOL. 2023 Aug;:117009Read more>>
  • Fei Hao. et al. Maternal exposure to deltamethrin during pregnancy and lactation impairs neurodevelopment of male offspring. ECOTOX ENVIRON SAFE. 2024 Apr;274:116196Read more>>
  • Xie Jiangang. et al. Exertional heat stroke-induced changes in gut microbiota cause cognitive impairment in mice. BMC MICROBIOL. 2024 Dec;24(1):1-13Read more>>
  • Linxiao Wang. et al. Lactobacillus rhamnosus GG improves cognitive impairments in mice with sepsis. PEERJ. 2024 May;12:e17427Read more>>
VALIDATION IMAGES

Mouse brain lysates; probed with TrkB(Y817) (2A1 ) Monoclonal Antibody (bsm-52213R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.


SH-SY-5Y cells were stained with TrkB(Y817) (2A1 ) Monoclonal Antibody (bsm-52213R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with TrkB (Tyr817) (2A1 ) Monoclonal Antibody (bsm-52213R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TrkB(Y817) (2A1) Monoclonal Antibody, Unconjugated (bsm-52213R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Spinal cord tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates probed with Phospho-TrkB (Tyr817) Monoclonal Antibody, Unconjugated (bsm-52213R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.