bsm-52174R [Primary Antibody]
Phospholipase C gamma 1 (85F11) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Phospholipase C gamma 1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5335

Swiss Prot: P19174

Source: Recombinant human Phospholipase C gamma 1 protein, around C-terminal 150aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Testis tissue lysates; Lane 6: Human A431 cell lysates; Lane 7: Human A549 cell lysates; Lane 8: Human MCF-7 cell lysates; Lane 9: Human HeLa cell lysates probed with PLCG1 Monoclonal Antibody, Unconjugated (bsm-52174R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.