bsm-52163R-BF680 [Conjugated Primary Antibody]
Histone H2A.X (Ser139) Recombinant Antibody, AbBy Fluor® 680 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: H2A.X

Modification Site: S139

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3014

Swiss Prot: P16104

Source: KLH conjugated Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Ser139:QA(p-S)QE.

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008].

Conjugation: AbBy Fluor® 680

Excitation/ Emission: 679nm/702nm

Size: 100ul

Concentration: Lot dependent

Applications: WB(1:300-5000)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zixuan Liu. et al. Polybrominated diphenyl ethers quinone exhibits neurotoxicity by inducing DNA damage, cell cycle arrest, apoptosis and p53-driven adaptive response in microglia BV2 cells. Toxicology. 2021 Jun;457:152807Read more>>
  • Zhang, Xin. et al. Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2. Cell Death Dis. 2022 Feb;13(2):1-15Read more>>
  • Shikai Liu. et al. On-Demand Generation of Peroxynitrite from an Integrated Two-Dimensional System for Enhanced Tumor Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Chen-Lin Yu. et al. Hernandonine-Mediated Autophagic Cell Death in Hepatocellular Carcinoma: Interplay of p53 and YAP Signaling Pathways. FREE RADICAL BIO MED. 2024 JuRead more>>
VALIDATION IMAGES