DATASHEET
Host:
Rabbit
Target Protein:
Cyclin E1(T77)
Modification Site:
T77
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
898
Swiss Prot:
P24864
Source:
Synthetic peptide derived from human Cyclin E1(T77), around 50-100aa (phospho T77).
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
VALIDATION IMAGES
SW480 cells were stained with Cyclin E1(T77) (4G10 ) Monoclonal Antibody (bsm-52146R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HepG2 cells were stained with Cyclin E1(T77) (4G10 ) Monoclonal Antibody (bsm-52146R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.