DATASHEET
Host:
Rabbit
Target Protein:
AKT1(T450)
Modification Site:
T450
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
207
Swiss Prot:
P31749
Source:
Synthetic peptide derived from human AKT1(T450), around 400-470aa (phospho T450).
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.
Conjugation:
Cy3
Excitation/ Emission:
512,550nm/570,615nm
Size:
100ul
Concentration:
Lot dependent
Applications:
WB(1:300-5000)
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.