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Host: Rabbit
Target Protein: Cyclin B2
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 9133
Swiss Prot: O95067
Source: Recombinant human Cyclin B2 protein, around N-terminal 150aa.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Hela; Lane 2: K562 lysates probed with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded mouse colon section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse testis section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.