Host: Rabbit
Target Protein: Cyclin B1
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 891
Swiss Prot: P14635
Source: Recombinant human Cyclin B1 protein, around N-terminal 150aa.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
- Cong Lan. et al. Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction. EBIOMEDICINE. 2022 Aug;82:104139Read more>>
- Shasha Liu. et al. Dietary flavonoid tectochrysin synergistically enhances cisplatin sensitivity on ovarian cancer cells through targeting the CDK1/cyclin B1 pathway. J FUNCT FOODS. 2024 Jun;117:106243Read more>>
- Qinbing Xue. et al. Lycorine (Lycoris radiata)a unique natural medicine on breast cancer. J CELL MOL MED. 2024 Aug;28(16):e70032Read more>>
Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Cyclin B1 (2E6) Monoclonal Antibody (bsm-52044R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnight Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Jurkat, 2: HeLa, 3: HT-29 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 55 kDa Observed MW: 55 kDa