bsm-52022M [Primary Antibody]
BCL-2 (2C4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: BCL-2

Clonality: Monoclonal

Isotype: IgG2b

Entrez Gene: 596

Swiss Prot: P10415

Source: Recombinant human BCL-2 protein, around 50-150aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. Read more>>
  • Hongyan Yu. et al. TBBPA rather than its main derivatives enhanced growth of endometrial cancer via p53 ubiquitination. J ENVIRON SCI-CHINA. 2022 DecRead more>>
  • Til Bahadur Thapa Magar. et al. Novel Chlorin e6-Curcumin Derivatives as a Potential Photosensitizer: Synthesis, Characterization, and Anticancer Activity. PHARMACEUTICS. 2023 Jun;15(6):1577Read more>>
  • Peng Ting. et al. Elucidation of the relationship between evodiamine-induced liver injury and CYP3A4-mediated metabolic activation by UPLC-MS/MS analysis. ANAL BIOANAL CHEM. 2023 Jul;:1-17Read more>>
  • Chenghao Jin. et al. Propofol synergizes with circAPBB2 to protect against hypoxia/reoxygenation\induced oxidative stress, inflammation, and apoptosis of human cardiomyocytes. IMMUN INFLAMM DIS. 2023 Aug; 11(8): e952Read more>>
  • Qifeng Shi. et al. PLC-CN-NFAT1 signaling-mediated A and IL-1 crosstalk synergistically promotes hippocampal neuronal damage. INT IMMUNOPHARMACOL. 2024 Jun;134:112259Read more>>
VALIDATION IMAGES

Jurkat cell lysate probed with BCL-2 (2C4) Monoclonal Antibody, Unconjugated (bsm-52022M) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.


Paraformaldehyde-fixed, paraffin embedded human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with BCL-2 (2C4) Monoclonal Antibody (bsm-52022M) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.