bsm-51733M [Primary Antibody]
EIF2AK2 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: EIF2AK2

Clonality: Monoclonal

Entrez Gene: 5610

Swiss Prot: P05198

Source: Recombinant human EIF2A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-2000，

Predicted Molecular Weight: 61


Predicted Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH/3T3 cell lysates probed with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-51733M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: K562 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH/3T3 cell lysates; Lane 5: HepG2 cell lysates probed with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-51733M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.