DATASHEET
Host:
Mouse
Target Protein:
c-MYC
Clonality:
Monoclonal
Entrez Gene:
4609
Swiss Prot:
P01106
Source:
Recombinant human MYC.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
PRODUCT SPECIFIC PUBLICATIONS
- Hongyan Yu. et al. TBBPA rather than its main derivatives enhanced growth of endometrial cancer via p53 ubiquitination. J ENVIRON SCI-CHINA. 2022 DecRead more>>
VALIDATION IMAGES
Lane 1: Hela cell lysates; Lane 2: K562 cell lysates; Lane 3: Jurkat cell lysates; Lane 4: Raji cell lysates; Lane 5: LNCaP cell lysates probed with c-MYC Monoclonal Antibody, Unconjugated (bsm-51652M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with c-MYC monoclonal antibody, unconjugated (bsm-51652M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.