bsm-51577M [Primary Antibody]
VCP Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: VCP

Clonality: Monoclonal

Entrez Gene: 7415

Swiss Prot: P55072

Source: Recombinant human VCP.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-2000，ICC=1:100-500，IF=1:100-500，

Predicted Molecular Weight: 97


Predicted Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Min Fu. et al. Mechanisms of Sodium/Iodide Symporter-Mediated Mammary Gland Iodine Compensation during Lactation. NUTRIENTS. 2022 Jan;14(17):3592Read more>>
VALIDATION IMAGES

Lane 1: MCF-7 cell lysates; Lane 2: U251 cell lysates; Lane 3: NIH/3T3 cell lysates; Lane 4: C6 cell lysates; Lane 5: Mouse Liver tissue lysates probed with VCP Monoclonal Antibody, Unconjugated (bsm-51577M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.