bsm-51566M [Primary Antibody]
YY1(Nuclear Loading Control) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: YY1(Nuclear Loading Control)

Clonality: Monoclonal

Isotype: IgG2a

Entrez Gene: 7528

Swiss Prot: P25490

Source: Recombinant human YY1 (His-tagged).

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions).
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-1000，IHC-P=1:25-50，IHC-F=1:25-50，

Predicted Molecular Weight: 46


Cross Reactive Species: Others

Predicted Cross Reactive Species: Human
Rat
Monkey

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Monkey brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Zebrafish brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4°C, DAB staining.


Lane 1: Daudi cell lysates; Lane 2: U251 cell lysates probed with YY1 Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: 293 cell lysates; Lane 2:C6 cell lysates; Lane 3: Hela cell lysates; Lane 4: U-251MG cell lysates probed with YY1 Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.