bsm-51558M [Primary Antibody]
UCHL1/PGP9.5 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: UCHL1/PGP9.5

Immunogen Range: 151-223/223


Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 7345

Swiss Prot: P09936

Source: KLH conjugated synthetic peptide derived from human UCHL1/PGP9.5

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-1000，IHC-P=1:25-50，

Predicted Molecular Weight: 25


Cross Reactive Species: Xenopus laevis

Predicted Cross Reactive Species: Human
Mouse
Rat
Cow
Pig
Monkey

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human astroglioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UCHL1/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:25 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UCHL1/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:25 overnight at 4°C, DAB staining.


Lane 1: 293T cell lysates; Lane 2: NCI-H1299 cell lysates; Lane 3: PC-12 cell lysates probed with UCHL1/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.