bsm-51555M [Primary Antibody]
Erk2 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Erk2

Immunogen Range: 101-200/360


Clonality: Monoclonal

Isotype: IgG1 k

Entrez Gene: 5594

Swiss Prot: P28482

Source: KLH conjugated synthetic peptide derived from human Erk2

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-2000,

Predicted Molecular Weight: 42


Cross Reactive Species: Xenopus laevis

Predicted Cross Reactive Species: Human
Mouse
Rat
Cow

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: MCF-7 cell lysates; Lane 2: HepG2 cell lysates; Lane 3: NCI-H292 cell lysates; Lane 4: Hela cell lysates; Lane 5: NIH/3T3 cell lysates; Lane 6: Neuro-2a cell lysates; Lane 7: C6 cell lysates probed with Erk2 Monoclonal Antibody, Unconjugated (bsm-51555M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: Hela-MAPK1 KO cell lysates probed with Erk2 Monoclonal Antibody, Unconjugated (bsm-51555M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.