bsm-51458M [Primary Antibody]
SUMO3 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: SUMO3

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 6612

Swiss Prot: P55854

Source: Recombinant GST fusion protein encoding human SUMO3.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-2000,

Predicted Molecular Weight: 11.6


Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HL-60 cell lysates; Lane 2: Jurkat cell lysates; Lane 3: 293T/17 cell lysates probed with SUMO3 Monoclonal Antibody, Unconjugated (bsm-51458M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.