DATASHEET
Host:
Mouse
Target Protein:
BAIAP2
Clonality:
Monoclonal
Isotype:
IgG2b
Entrez Gene:
10458
Swiss Prot:
Q9UQB8
Source:
Purified His-tagged BAIAP2 protein was used to produced this monoclonal antibody.
Purification:
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Participates in actin bundling when associated with EPS8, promoting filopodial protrusions.
VALIDATION IMAGES
Lane 1: K562 Cell lysates; Lane 2: mouse brain lysates; Lane 3: U-251 MG Cell lysates; probed with BAIAP2 (1037CT15.1.3) Monoclonal Antibody, unconjugated (bsm-51410M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Cerebrum lysates probed with BAIAP2 (4G7) Monoclonal Antibody, Unconjugated (bsm-51410M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.