bsm-51404M [Primary Antibody]
USP5 (13C4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: USP5

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 8078

Swiss Prot: P45974

Source: This USP5 antibody is generated from a mouse immunized with a recombination protein from the human region of human USP5.

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 96


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: mouse brain lysates; Lane 2: PC-3 Cell lysates; Lane 3: rat liver Cell lysates; Lane 4: U-251 MG Cell lysates; Lane 5: rat brain Cell lysates; probed with USP5 (13C4)) Monoclonal Antibody, unconjugated (bsm-51404M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.