bsm-51402M [Primary Antibody]
RAD51 (1C9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: RAD51

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 5888

Swiss Prot: Q06609

Source: This RAD51 antibody is generated from a mouse immunized with a recombination protein from human.

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 37


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chien Jasper Che-Yung. et al. A multiplexed bioluminescent reporter for sensitive and non-invasive tracking of DNA double strand break repair dynamics in vitro and in vivo. Nucleic Acids Res. 2020 Sep;48(17):e100-e1Read more>>
VALIDATION IMAGES

Lane 1: Jurkat Cell lysates; Lane 2: NIH/3T3 Cell lysates; Lane 3: C6 Cell lysates; Lane 4: PC-12 Cell lysates; Lane 5: Hela Cell lysates; probed with RAD51 (1C9) Monoclonal Antibody, unconjugated (bsm-51402M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Raji cell lysates probed with RAD5 Monoclonal Antibody, Unconjugated (bsm-51402M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.