bsm-51379M [Primary Antibody]
MB21D1 (7E3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: MB21D1

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 115004

Swiss Prot: Q8N884

Source: This MB21D1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-185 amino acids from human MB21D1.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. cGAMP can be transferred between cells by virtue of packaging within viral particles contributing to IFN-induction in newly infected cells in a cGAS-independent but TMEM173/STING-dependent manner (PubMed:26229115).

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 59


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiaofei Wang. et al. Selenomethionine mitigate PM2.5-induced cellular senescence in the lung via attenuating inflammatory response mediated by cGAS/STING/NF-B pathway. ECOTOX ENVIRON SAFE. 2022 Dec;247:114266Read more>>
VALIDATION IMAGES

Lane 1: THP-1; Lane 2: MDA-MB-31 cell lysate at 20 µg per lane, probed with bsm-51379M MB21D1 (7E3) Monoclonal Antibody at 1:4000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.