DATASHEET
Host:
Mouse
Target Protein:
CYLD
Clonality:
Monoclonal
Isotype:
IgG2a
Entrez Gene:
1540
Swiss Prot:
Q9NQC7
Source:
This CYLD antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 305-582 amino acids from human CYLD.
Purification:
Purified by Protein G.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation (PubMed:12917689, PubMed:12917691). Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation (PubMed:12917690). Negative regulator of Wnt signaling (PubMed:20227366). Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules (PubMed:19893491). Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis (PubMed:18222923, PubMed:20194890). Required for normal cell cycle progress and normal cytokinesis (PubMed:17495026, PubMed:19893491). Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation (PubMed:18636086). Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis (By similarity). Involved in the regulation of ciliogenesis, allowing ciliary basal bodies to migrate and dock to the plasma membrane; this process does not depend on NF-kappa-B activation (By similarity).
VALIDATION IMAGES
Lane 1: HeLa Cell lysates; probed with CYLD (7C1) Monoclonal Antibody, unconjugated (bsm-51375M) at 1:4000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Hela probed with using CYLD (7C1) Monoclonal Antibody (green) unconjugated bsm-51375M at 1:25 for 60 minutes at 37ºC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG2a (blue).
25 ug total protein per lane of various lysates (see on figure) probed with CYLD monoclonal antibody, unconjugated (bsm-51375M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.