bsm-51351M [Primary Antibody]
CDH1 (9C8) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: CDH1

Immunogen Range: 1-392/882


Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 999

Swiss Prot: P12830

Source: This CADH1 antibody is generated from a mouse immunized with a recombinat protein between 1-392 amino acids from human CADH1.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 97


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: A431 Cell lysates; Lane 2: T47D Cell lysates; probed with CDH1 (9C8) Monoclonal Antibody, unconjugated (bsm-51351M) at 1:4000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded human colon tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDH1 (9C8) Monoclonal Antibody (bsm-51351M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.


Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized MCF-7 cells; antibody incubation with CDH1 (9C8) Monoclonal Antibody, Unconjugated (bsm-51351M) at 1:25 overnight at 4°C, followed by a conjugated secondary DAPI and for nuclei staining.