Host: Mouse
Target Protein: GARS
Clonality: Monoclonal
Isotype: IgG1
Entrez Gene: 2617
Swiss Prot: P41250
Source: This GARS antibody is generated from a mouse immunized with a recombinant protein between 15-305 amino acids from human GARS.
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 0.5ug/ul
Predicted Molecular Weight: 83
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Jurkat Cell lysates; Lane 2: Ramos Cell lysates; Lane 3: HT-1080 Cell lysates; probed with GARS (1C6) Monoclonal Antibody, unconjugated (bsm-51350M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells; antibody incubation with GARS (1C6) Monoclonal Antibody, Unconjugated (bsm-51350M) at 1:25 overnight at 4°C, followed by a conjugated secondary (green). Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Hela cells probed with using GARS (1C6) Monoclonal Antibody (green) unconjugated bsm-51350M at 1:25 for 60 minutes at 37ºC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).