bsm-51338M [Primary Antibody]
PDIA1 (3C3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: PDIA1

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 5034

Swiss Prot: P07237

Source: This PDIA1 antibody is generated from a mouse immunized with a recombinant protein of human PDIA1.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 57


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HeLa; Lane 2: MCF-7; Lane 3: HepG2; Lane 4: PANC-1 cell testis lysate (20µg) probed with bsm-51338M PDIA1 (3C3) Monoclonal Antibody at 1:4000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.